avi tag sequence

Understanding Avi-Tagged Proteins

Avi-tagged proteins are a powerful tool in protein engineering, used to introduce a site-specific biotin label into a protein. The Avi tag is typically fused to either the the N-terminus or C-terminus of a target protein, and then enzymatically biotinylated.

If you wish to see the detailed description from Beta LifeScience, you can check out: avi tag sequence.

Applications & Use Cases

Some common applications of Avi-tagged proteins include:

• Protein purification & immobilization
  Biotinylated proteins can be captured using monomeric avidin or avidin derivatives, immobilized on various surfaces (plates, chips, beads) for assays.

• Protein detection
  

  After binding with streptavidin or avidin conjugates, the protein can be detected in Western blots, ELISAs, or cell assays.

• Best Practices & Considerations

  • Choice of tag location — Decide whether N- or C-terminal fusion is optimal so as not to interfere with active domains.

  • Ensuring complete labeling — Sometimes in vitro biotinylation with BirA is used to guarantee full modification.

  • Validation — Confirm successful biotinylation using streptavidin blots or mass spectrometry.

  • Purification strategy — Use low-affinity monomeric avidin or anti-biotin affinity media to recover biotinylated protein.

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